CNOT7 Polyklonaler Antikörper

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• KD/KO Validated

CNOT7 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14102-1-AP

Synonyme

BTG1 binding factor 1, CAF 1, CAF1, CCR4 associated factor 1, CNOT7, hCAF 1

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 14102-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14102-1-AP (CNOT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14102-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14102-1-AP (CNOT7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14102-1-AP

  IP Result of anti-CNOT7 (IP:14102-1-AP, 4ug; Detection:14102-1-AP 1:300) with mouse testis tissue lysate 4800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

14102-1-AP bindet in WB, IP, ELISA CNOT7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNOT7 fusion protein Ag5235
• Vollständiger Name: CCR4-NOT transcription complex, subunit 7
• Berechnetes Molekulargewicht: 285 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC060852
• Gene symbol: CNOT7
• Gene ID (NCBI): 29883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved inglobal gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].

Publikationen

Species	Application	Title
human	WB	Nature; DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.; Authors - Masashi Yamaji
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	J Inflamm (Lond); The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.; Authors - Hsin-Hui Hsieh ; KD Validated
		Int J Mol Med; B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis.; Authors - Guogui Sun
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan
human	WB	Nucleic Acids Res; BESST: a novel LncRNA knockout strategy with less genome perturbance; Authors - Shikuan Zhang