CNDP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CNDP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14925-1-AP

Synonyme

CN2, CNDP dipeptidase 2, CNDP2, CPGL, HsT2298, PEPA, Peptidase A

---

Galerie der Validierungsdaten

• 

  WB analysis using 14925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14925-1-AP (CNDP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14925-1-AP

  IP result of anti-CNDP2 (IP:14925-1-AP, 4ug; Detection:14925-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

• 

  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-3 using 14925-1-AP

  Immunofluorescent analysis of PC-3 cells using 14925-1-AP (CNDP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, LNCaP-Zellen, Mausnierengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14925-1-AP bindet in WB, IP, IHC, IF, ELISA CNDP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNDP2 fusion protein Ag6684
• Vollständiger Name: CNDP dipeptidase 2 (metallopeptidase M20 family)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 44-53 kDa
• GenBank-Zugangsnummer: BC001375
• Gene symbol: CNDP2
• Gene ID (NCBI): 55748
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa. The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

Publikationen

Species	Application	Title
	IHC	Acta Neuropathol; The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.; Authors - David C Hondius
mouse	WB	Cell Chem Biol; Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.; Authors - Joon T Kim ; KO Validated
human	WB,IHC	Mol Med; Underexpressed CNDP2 participates in gastric cancer growth inhibition through activating the MAPK signaling pathway.; Authors - Zhenwei Zhang
human	WB,IF	BMC Cancer; The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study; Authors - Ying E
human	WB	PLoS One; Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.; Authors - Li Li
human	WB,IHC	BMC Gastroenterol; Up-regulation of CNDP2 facilitates the proliferation of colon cancer.; Authors - Conglong Xue ; KD Validated