CNBP Monoklonaler Antikörper

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• KD/KO Validated

CNBP Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1H1D12

Rezensionen (1)

Kat-Nr. : 67109-1-Ig

Synonyme

CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

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Galerie der Validierungsdaten

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  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67109-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67109-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67109-1-Ig

  IP result of anti-CNBP (IP:67109-1-Ig, 5ug; Detection:67109-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, A549-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-29-Zellen, Jurkat-Zellen, NCI-H1299-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

67109-1-Ig bindet in WB, IP, ELISA CNBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CNBP fusion protein Ag7114
• Vollständiger Name: CCHC-type zinc finger, nucleic acid binding protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19-25 kDa
• GenBank-Zugangsnummer: BC000288
• Gene symbol: CNBP
• Gene ID (NCBI): 7555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP,WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

The band was clear

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T