CMPK2 Polyklonaler Antikörper

CMPK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25877-1-AP

Synonyme

EC:2.7.4.14, EC:2.7.4.6, Nucleoside-diphosphate kinase, TYKi, UMP CMPK2

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  WB analysis using 25877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25877-1-AP (CMPK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 25877-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25877-1-AP (CMPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

25877-1-AP bindet in WB, IHC, ELISA CMPK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CMPK2 fusion protein Ag22885
• Vollständiger Name: cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial
• Berechnetes Molekulargewicht: 449 aa, 49 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC141802
• Gene symbol: CMPK2
• Gene ID (NCBI): 129607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CMPK2 belongs to the thymidylate kinase family. CMPK2 may participate in dUTP and dCTP synthesis in mitochondria. It is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy of CMPK2 is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs.

Publikationen

Species	Application	Title
human	IHC	Front Genet; Mitochondrial-related hub genes in dermatomyositis: muscle and skin datasets-based identification and in vivo validation; Authors - Shuo Wang
human	WB	Sci Rep; LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes; Authors - Jianbing Hao
human	WB	Cell Biochem Biophys; CMPK2 Promotes CD4+ T Cell Activation and Apoptosis through Modulation of Mitochondrial Dysfunction in Systemic Lupus Erythematosus; Authors - Ya-Nan Tan ; KD Validated
mouse	IHC	Int J Med Sci; Discovery and confirmation of crucial genes associated with radiation-induced heart disease; Authors - Chuanbin Liu
mouse	WB	Int J Biol Sci; The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation; Authors - Li Jin