Mast Cell Chymase Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Mast Cell Chymase Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 18189-1-AP

Synonyme

Alpha chymase, chymase, chymase 1, mast cell, CMA1, CYH, CYM, Mast Cell Chymase, Mast cell protease I, Mcpt 5, Mcpt5, MCT1

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 18189-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18189-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18189-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18189-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 18189-1-AP

  IP Result of anti-Mast Cell Chymase (IP:18189-1-AP, 4ug; Detection:18189-1-AP 1:300) with mouse heart tissue lysate 2400ug.

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  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Produktinformation

18189-1-AP bindet in WB, IP, IHC, IF, ELISA Mast Cell Chymase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mast Cell Chymase fusion protein Ag12909
• Vollständiger Name: chymase 1, mast cell
• Berechnetes Molekulargewicht: 247 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC069370
• Gene symbol: CMA1
• Gene ID (NCBI): 1215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.; Authors - Emily Mackey
human	WB	Arterioscler Thromb Vasc Biol; Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide).; Authors - Wen Chen
human		Int J Mol Sci; Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.; Authors - Mika Yamanaka-Takaichi
human	WB	Cell Cycle; Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.; Authors - Haoqing Shi
mouse	WB	Sci Rep; Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.; Authors - Nicole Meyer
mouse	WB	Immunology; The circadian clock is functional in eosinophils and mast cells.; Authors - Baumann Anja A