CLPB Polyklonaler Antikörper

CLPB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15743-1-AP

Synonyme

CLPB, HSP78, SKD3

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Galerie der Validierungsdaten

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  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 15743-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLPB antibody (15743-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

15743-1-AP bindet in WB, IHC, IF, ELISA CLPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLPB fusion protein Ag8165
• Vollständiger Name: ClpB caseinolytic peptidase B homolog (E. coli)
• Berechnetes Molekulargewicht: 707 aa, 79 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC006404
• Gene symbol: CLPB
• Gene ID (NCBI): 81570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260)

Publikationen

Species	Application	Title
	WB	Sci Adv; Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding; Authors - Arpit Gupta
human	WB	Elife; Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations.; Authors - Ryan R Cupo
human	WB	iScience; Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region.; Authors - Takahiro Yoshinaka
mouse	WB	Elife; Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis; Authors - Enrico Radaelli