CLOCK Polyklonaler Antikörper

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• KD/KO Validated

CLOCK Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 18094-1-AP

Synonyme

bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 18094-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18094-1-AP

  IP result of anti-CLOCK (IP:18094-1-AP, 4ug; Detection:18094-1-AP 1:300) with HeLa cells lysate 1600 ug.

• 

  IF Staining of HeLa using 18094-1-AP

  Immunofluorescent analysis of HeLa cells using 18094-1-AP (CLOCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18094-1-AP bindet in WB, IHC, IF, ELISA CLOCK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLOCK fusion protein Ag12826
• Vollständiger Name: clock homolog (mouse)
• Berechnetes Molekulargewicht: 846 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95-110 kDa
• GenBank-Zugangsnummer: BC041878
• Gene symbol: CLOCK
• Gene ID (NCBI): 9575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
human	WB	Int J Mol Sci; Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.; Authors - Weiyun Zhang ; KD Validated
mouse	WB	J Agric Food Chem; 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2; Authors - Shuang Guan
human	WB, IHC	Neoplasia; A long noncoding RNA perturbs the circadian rhythm of hepatoma cells to facilitate hepatocarcinogenesis.; Authors - Ming Cui
human	WB	J Agric Food Chem; Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.; Authors - Run Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Verdiana (Verified Customer) (05-03-2022)

The bands are clear, there is no background noise even if the bands signal is not very strong. Good Ab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastic cell line