CLIP-115/CLIP2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CLIP-115/CLIP2 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6B6

Kat-Nr. : 67776-1-Ig

Synonyme

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

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Galerie der Validierungsdaten

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  WB analysis of mouse cerebellum using 67776-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67776-1-Ig

  WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

• 

  WB analysis of rat cerebellum using 67776-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 67776-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67776-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67776-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67776-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67776-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67776-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, A549-Zellen, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67776-1-Ig bindet in WB, IF, ELISA CLIP-115/CLIP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLIP-115/CLIP2 fusion protein Ag18902
• Vollständiger Name: CAP-GLY domain containing linker protein 2
• Berechnetes Molekulargewicht: 1011 aa, 112 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC150332
• Gene symbol: CLIP2
• Gene ID (NCBI): 7461
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.