CLIC4 Monoklonaler Antikörper

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• KD/KO Validated

CLIC4 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B7F9

Kat-Nr. : 66343-1-Ig

Synonyme

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66343-1-Ig

  WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

• 

  WB analysis of human heart using 66343-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66343-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 66343-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 66343-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Hausschwein-Herzgewebe, Hausschwein-Nierengewebe, HeLa-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66343-1-Ig bindet in WB, IHC, IF, ELISA CLIC4 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLIC4 fusion protein Ag2942
• Vollständiger Name: chloride intracellular channel 4
• Berechnetes Molekulargewicht: 253 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC012444
• Gene symbol: CLIC4
• Gene ID (NCBI): 25932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Publikationen

Species	Application	Title
rat	WB,IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li