CLEC14A Polyklonaler Antikörper

CLEC14A Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23964-1-AP

Synonyme

C14orf27, CEG1, CLEC14A

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Galerie der Validierungsdaten

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  WB analysis of human heart using 23964-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23964-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 23964-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 23964-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug CLEC14A antibody (23964-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

23964-1-AP bindet in WB, IHC, FC, ELISA CLEC14A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLEC14A fusion protein Ag21081
• Vollständiger Name: C-type lectin domain family 14, member A
• Berechnetes Molekulargewicht: 490 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC031567
• Gene symbol: CLEC14A
• Gene ID (NCBI): 161198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	FASEB J; CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.; Authors - Zeyu Su