CKM/CKB Polyklonaler Antikörper

CKM/CKB Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15891-1-AP

Synonyme

CKM, CKM/CKB, CKMM, Creatine Kinase, Creatine Kinase MM, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 15891-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15891-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15891-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 15891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 15891-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15891-1-AP bindet in WB, IHC, ELISA CKM/CKB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKM/CKB fusion protein Ag8678
• Vollständiger Name: creatine kinase, muscle
• Berechnetes Molekulargewicht: 381 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC007462
• Gene symbol: CKM
• Gene ID (NCBI): 1158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.

Publikationen

Species	Application	Title
rat	WB	Lipids Health Dis; Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.; Authors - David W Russ
human	WB	Spinal Cord; The relationship between skin ultrasound images and muscle damage using skin blotting in wheelchair basketball athletes.; Authors - Nao Tamai
human	WB	Nanomedicine; Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.; Authors - Adel Al Fatease