CHST3 Polyklonaler Antikörper

CHST3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18242-1-AP

Synonyme

C6ST, C6ST 1, C6ST1, Chondroitin 6 sulfotransferase, CHST3, GST 0, HSD

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

18242-1-AP bindet in WB, IHC, IF, ELISA CHST3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHST3 fusion protein Ag13008
• Vollständiger Name: carbohydrate (chondroitin 6) sulfotransferase 3
• Berechnetes Molekulargewicht: 479 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-67 kDa
• GenBank-Zugangsnummer: BC093690
• Gene symbol: CHST3
• Gene ID (NCBI): 9469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.; Authors - Laura Hobohm
human	IHC	Osteoarthritis Cartilage; Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.; Authors - Jing Han
mouse	IF	Cells; Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.; Authors - David Baidoe-Ansah
mouse	IHC	J Neurochem; OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex.; Authors - Hiroaki Okuda
human	IHC	Histochem Cell Biol; Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.; Authors - Jian Lei
human	WB	Biol Trace Elem Res; Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.; Authors - Liyun Wang