CHRNA7 Polyklonaler Antikörper

CHRNA7 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 21379-1-AP

Synonyme

CHRNA7, CHRNA7 2, NACHRA7

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Galerie der Validierungsdaten

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  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21379-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21379-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 21379-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNA7 antibody (21379-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

21379-1-AP bindet in WB, IHC, IF, FC, ELISA CHRNA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNA7 fusion protein Ag15980
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 7
• Berechnetes Molekulargewicht: 502 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC037571
• Gene symbol: CHRNA7
• Gene ID (NCBI): 1139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.

Publikationen

Species	Application	Title
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
human	WB	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang
mouse	WB,IF	Front Pharmacol; Autophagy is Involved in Neuroprotective Effect of Alpha7 Nicotinic Acetylcholine Receptor on Ischemic Stroke.; Authors - Zhe-Qi Xu
rat	WB,IF	Front Pharmacol; Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson's Disease Rats.; Authors - Li Zhou
mouse	WB	J Alzheimers Dis; Gx-50 Inhibits Neuroinflammation via α7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways; Authors - Shi Shi
human,mouse	WB,IF	Mol Med; α7 nicotinic acetylcholine receptor agonist GTS-21 attenuates DSS-induced intestinal colitis by improving intestinal mucosal barrier function.; Authors - Ziping Ye

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