CHRNA5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CHRNA5 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

3G8C11

Kat-Nr. : 66363-1-Ig

Synonyme

CHRNA5, LNCR2, NACHRA5

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse skeletal muscle using 66363-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 66363-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66363-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 66363-1-Ig

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66363-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human bladder using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human bladder using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, A549-Zellen, Hausschwein-Skelettmuskelgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Blasengewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 8 publications below

Produktinformation

66363-1-Ig bindet in WB, IHC, ELISA CHRNA5 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHRNA5 fusion protein Ag4447
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 5
• Berechnetes Molekulargewicht: 468 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC033639
• Gene symbol: CHRNA5
• Gene ID (NCBI): 1138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Cell Mol Life Sci; Acetylcholine promotes chronic stress-induced lung adenocarcinoma progression via α5-nAChR/FHIT pathway; Authors - Yang Jiao ; KD Validated
human,mouse	IHC	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang ; KD Validated
human	IHC	Carcinogenesis; α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.; Authors - Qian Zhang
human	IHC	Mol Carcinog; PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma; Authors - Qiang Li
human	IHC	Toxicology; The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration.; Authors - Ying Jia
human	IHC	Hum Cell; The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion.; Authors - Ping Zhu