CHPF N-terminal Polyklonaler Antikörper

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• KD/KO Validated

CHPF N-terminal Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 23953-1-AP

Synonyme

Chondroitin sulfate synthase 2, CHPF, CHPF N-terminal, CHSY2, CSS2

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Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 23953-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23953-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 23953-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23953-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, C6-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Kolongewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

23953-1-AP bindet in WB, IHC, IF, ELISA CHPF N-terminal und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHPF N-terminal fusion protein Ag20819
• Vollständiger Name: chondroitin polymerizing factor
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC023531
• Gene symbol: CHPF
• Gene ID (NCBI): 79586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cells; Targeting Chondroitin Sulfate Reduces Invasiveness of Glioma Cells by Suppressing CD44 and Integrin β1 Expression.; Authors - Yin-Hung Chu
human	IHC,WB	J Cell Commun Signal; Chondroitin polymerizing factor predicts a poor prognosis and promotes breast cancer progression via the upstream TGF-β1/SMAD3 and JNK axis activation; Authors - Qiang-Feng Pan
human	WB,IHC,IF	Aging (Albany NY); The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma; Authors - Haitao Luo ; KD Validated