CHOP; GADD153 Monoklonaler Antikörper

CHOP; GADD153 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

CloneNo.

4D5A9

Kat-Nr. : 60304-1-Ig

Synonyme

CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 60304-1-Ig

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60304-1-Ig (CHOP; GADD153 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Tunicamycin behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60304-1-Ig bindet in WB, IHC, IF, ELISA CHOP; GADD153 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHOP; GADD153 fusion protein Ag7354
• Vollständiger Name: DNA-damage-inducible transcript 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003637
• Gene symbol: DDIT3
• Gene ID (NCBI): 1649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publikationen

Species	Application	Title
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang
human	WB,IHC	Cell Death Dis; YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.; Authors - Adrian Rivera-Reyes
rat	WB	Hum Mol Genet; The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa.; Authors - Dimitra Athanasiou
human, mouse	WB	Oncotarget; Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.; Authors - Valeria Catena
human	WB	Apoptosis; Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation.; Authors - Xiaoli Liu
human	WB	J Virol; ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress; Authors - Mengtian Cui