CHKA-Specific Polyklonaler Antikörper

CHKA-Specific Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19537-1-AP

Synonyme

CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 19537-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19537-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19537-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of HepG2 using 19537-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHKA antibody 19537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, humanes Hodengewebe, Maushodengewebe, Maus-Kolongewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19537-1-AP bindet in WB, IHC, IF,ELISA CHKA-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: choline kinase alpha
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_001277
• Gene symbol: CHKA
• Gene ID (NCBI): 1119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.