Chk1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Chk1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 25887-1-AP

Synonyme

CHEK1, CHK1

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Galerie der Validierungsdaten

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  WB analysis using 25887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 25887-1-AP

  IP Result of anti-CHK1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug.

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  IHC staining of human kidney using 25887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293T using 25887-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 25887-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293T-Zellen
• Erfolgreiche Detektion in FC: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

25887-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA Chk1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chk1 fusion protein Ag22993
• Vollständiger Name: CHK1 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC004202
• Gene symbol: CHEK1
• Gene ID (NCBI): 1111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability; Authors - Zaili Luo
human	WB	Nat Commun; G-quadruplex DNA structures in human stem cells and differentiation.; Authors - Katherine G Zyner
human	WB	Cell Death Differ; Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.; Authors - Yan Zhao
human	WB	Cancer Res; Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.; Authors - Zhe Sun
human	WB	Clin Cancer Res; RNF126 as a biomarker of a poor prognosis in invasive breast cancer and CHK1 inhibitor efficacy in breast cancer cells.; Authors - Xiaosong Yang
human	WB	Haematologica; The HDAC and PI3K dual inhibitor CUDC-907 synergistically enhances the antileukemic activity of venetoclax in preclinical models of acute myeloid leukemia.; Authors - Xinyu Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k. (Verified Customer) (10-26-2023)

The antibody worked for WB at a 1:100 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

Chk1 Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:25887-1-AP)

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011