CFTR Polyklonaler Antikörper

CFTR Polyklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20738-1-AP

Synonyme

ABC35, ABCC7, CF, CFTR, CFTR/MRP, MRP7, TNR CFTR



Geprüfte Anwendungen

Erfolgreiche Detektion in IHChumanes Lungengewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20738-1-AP bindet in WB, IHC, IF, FC, ELISA CFTR und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Berechnetes Molekulargewicht 168 kDa
GenBank-ZugangsnummerNM_000492
Gene symbol CFTR
Gene ID (NCBI) 1080
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

      What is the human cytogenetic location of the CFTR gene?

7q31.2

 

What is the predicted molecular weight of CFTR? 

168 kDa

 

Where is CFTR expressed?

CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.

 

What is the ion selectivity of CFTR?

CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.

 

What is the process of CFTR trafficking?

The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.

 

What are the separate domains of CFTR?

CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.

 

What regulates CFTR?

CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.

 

What is the role of CFTR?

CFTR functions as a channel for anions, regulating water and ionic homeostasis.

 

What mutations in CFTR leads to protein degradation?

Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.

 

How does CFTR differ from other ATP-binding cassette proteins?

Unlike other proteins in the ABC family, CFTR functions as an ion channel.


PMIDs: 24513531, 24534272, 22966013, 22709980


Protokolle

Produktspezifische Protokolle
IHC protocol for CFTR antibody 20738-1-APProtokoll herunterladen
IF protocol for CFTR antibody 20738-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
humanIF

PLoS One

Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.

Authors - Mauricio Di Fulvio
humanWB

J Exp Clin Cancer Res

Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer

Authors - Fangyuan Zhou
humanIHC

Int Immunopharmacol

A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma

Authors - Xing Huang
mouseWB

Acta Pharmacol Sin

Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation

Authors - Hui-Fang Zhang