CFTR Polyklonaler Antikörper
CFTR Polyklonal Antikörper für FC, IF, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20738-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IHC | humanes Lungengewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen |
Erfolgreiche Detektion in FC | HUVEC-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Produktinformation
20738-1-AP bindet in WB, IHC, IF, FC, ELISA CFTR und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) |
Berechnetes Molekulargewicht | 168 kDa |
GenBank-Zugangsnummer | NM_000492 |
Gene symbol | CFTR |
Gene ID (NCBI) | 1080 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
What is the human cytogenetic location of the CFTR gene?
7q31.2
What is the predicted molecular weight of CFTR?
168 kDa
Where is CFTR expressed?
CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.
What is the ion selectivity of CFTR?
CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.
What is the process of CFTR trafficking?
The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.
What are the separate domains of CFTR?
CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.
What regulates CFTR?
CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.
What is the role of CFTR?
CFTR functions as a channel for anions, regulating water and ionic homeostasis.
What mutations in CFTR leads to protein degradation?
Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.
How does CFTR differ from other ATP-binding cassette proteins?
Unlike other proteins in the ABC family, CFTR functions as an ion channel.
PMIDs: 24513531, 24534272, 22966013, 22709980
Protokolle
Produktspezifische Protokolle | |
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IHC protocol for CFTR antibody 20738-1-AP | Protokoll herunterladen |
IF protocol for CFTR antibody 20738-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
PLoS One Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet. | ||
J Exp Clin Cancer Res Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer | ||
Int Immunopharmacol A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma | ||
Acta Pharmacol Sin Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation |