CEP70 Polyklonaler Antikörper

CEP70 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16280-1-AP

Synonyme

BITE, centrosomal protein 70kDa, Centrosomal protein of 70 kDa, CEP70, p10 binding protein

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 16280-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16280-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16280-1-AP (CEP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Maushodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

16280-1-AP bindet in WB, IF, ELISA CEP70 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP70 fusion protein Ag9365
• Vollständiger Name: centrosomal protein 70kDa
• Berechnetes Molekulargewicht: 597 aa, 70 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC016050
• Gene symbol: CEP70
• Gene ID (NCBI): 80321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.

Publikationen

Species	Application	Title
human,mouse	IF	Front Endocrinol (Lausanne); Identification of biallelic variations of CEP70 in patients with male infertility; Authors - Tiechao Ruan