CEP63 Polyklonaler Antikörper

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• KD/KO Validated

CEP63 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 16268-1-AP

Synonyme

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 16268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16268-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16268-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16268-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16268-1-AP

  IP Result of anti-CEP63 (IP:16268-1-AP, 3ug; Detection:16268-1-AP 1:500) with HEK-293 cells lysate 1700ug.

• 

  IF Staining of MDCK using 16268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP63 antibody (16268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mauslebergewebe, Maus-Thymusgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 9 publications below
• IP: See 3 publications below

Produktinformation

16268-1-AP bindet in WB, IP, IF, ELISA CEP63 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP63 fusion protein Ag9335
• Vollständiger Name: centrosomal protein 63kDa
• Berechnetes Molekulargewicht: 703 aa, 81 kDa
• Beobachtetes Molekulargewicht: 57-75 kDa
• GenBank-Zugangsnummer: BC014050
• Gene symbol: CEP63
• Gene ID (NCBI): 80254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

Publikationen

Species	Application	Title
mouse		Nat Cell Biol; Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.; Authors - Olivier Mercey
mouse	WB,IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
human	WB, IF, IP	Dev Cell; A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.; Authors - Zhen Zhao ; KO Validated
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
human	IF	Nat Commun; Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.; Authors - Søs Grønbæk Holdgaard
mouse	IF	Nat Commun; Autophagy controls centrosome number by degrading Cep63.; Authors - Yuichiro Watanabe