CEP290 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CEP290 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 22490-1-AP

Synonyme

3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 22490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22490-1-AP

  IP result of anti-CEP290(IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22490-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Produktinformation

22490-1-AP bindet in WB, IP, IHC, IF, ELISA CEP290 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP290 fusion protein Ag17457
• Vollständiger Name: centrosomal protein 290kDa
• Berechnetes Molekulargewicht: 2479 aa, 290 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: BC008641
• Gene symbol: CEP290
• Gene ID (NCBI): 80184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.

Publikationen

Species	Application	Title
mouse	IF	J Cell Biol; The small GTPase RSG1 controls a final step in primary cilia initiation.; Authors - Stephanie O Agbu
mouse	WB	Cell Rep; A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.; Authors - Suddhasil Mookherjee
human	IF	EMBO Rep; Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.; Authors - Andrea Martello
human	WB,IHC,IP	Aging (Albany NY); Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.; Authors - Yiru Shan ; KD Validated
human	WB,IF	J Biol Chem; DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.; Authors - Boyan Zhang
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixation.Immunostaining result shows that CEP290 localizes to centrosome (Red; CEP290, Green; CEP164 for Centrosome marker, Blue;DAPI for Nucleus).The antibody seems to detect additional nucleoli signals.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP290 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:22490-1-AP)