CEP135 Polyklonaler Antikörper

CEP135 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 24428-1-AP

Synonyme

centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635

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Galerie der Validierungsdaten

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  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, U2OS-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 5 publications below

Produktinformation

24428-1-AP bindet in WB, IF, ELISA CEP135 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP135 fusion protein Ag19122
• Vollständiger Name: centrosomal protein 135kDa
• Berechnetes Molekulargewicht: 1140 aa, 134 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: BC136535
• Gene symbol: CEP135
• Gene ID (NCBI): 9662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human		Elife; WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.; Authors - Emmanuelle Steib
human	IF	Cells; Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.; Authors - Zhenzhen Chu
human	IF	Mol Biol Cell; The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.; Authors - Alexander J Stemm-Wolf
human	IF	Gene; A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).; Authors - Yan-Wei Sha

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (01-25-2023)

RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CEP135 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24428-1-AP)