CENPF Polyklonaler Antikörper

CENPF Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-28568

Synonyme

AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779

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Galerie der Validierungsdaten

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  IF Staining of A549 using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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  IF Staining of HeLa using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-28568 bindet in IF CENPF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPF fusion protein Ag29665
• Vollständiger Name: centromere protein F, 350/400ka (mitosin)
• Berechnetes Molekulargewicht: 368 kDa
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: NM_016343
• Gene symbol: CENPF
• Gene ID (NCBI): 1063
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).