CENPE Polyklonaler Antikörper

CENPE Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 28142-1-AP

Synonyme

CENP E, CENPE, Centromere protein E, centromere protein E, 312kDa, KIF10, Kinesin related protein CENPE

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Galerie der Validierungsdaten

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  WB analysis using 28142-1-AP

  HEK-293 cells lysate were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 28142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 28142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

28142-1-AP bindet in WB, IHC, ELISA CENPE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPE fusion protein Ag27230
• Vollständiger Name: centromere protein E, 312kDa
• Berechnetes Molekulargewicht: 316 kDa
• Beobachtetes Molekulargewicht: 300-316 kDa
• GenBank-Zugangsnummer: NM_001286734
• Gene symbol: CENPE
• Gene ID (NCBI): 1062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Biochim Biophys Acta Mol Cell Res; Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes.; Authors - Zhen-Yu She
human	WB	Am J Transl Res; Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.; Authors - Xishan Li
human	IHC	PLoS One; CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival.; Authors - Xueqiang Zhu
mouse	WB	Cell Mol Life Sci; Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse; Authors - Haojie Li
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi