CENPA Polyklonaler Antikörper

CENPA Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 26754-1-AP

Synonyme

CENP A, CENPA, Centromere autoantigen A, centromere protein A

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 26754-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26754-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

26754-1-AP bindet in WB, IHC, ELISA CENPA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPA fusion protein Ag25025
• Vollständiger Name: centromere protein A
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC002703
• Gene symbol: CENPA
• Gene ID (NCBI): 1058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Publikationen

Species	Application	Title
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi