CDKAL1 Polyklonaler Antikörper

CDKAL1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22988-1-AP

Synonyme

CDKAL1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse lung using 22988-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22988-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 22988-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22988-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22988-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 22988-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus slide using 22988-1-AP (CDKAL1 Antibody) at dilution of 1:50.

• 

  IHC staining of human oesophagus using 22988-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus slide using 22988-1-AP (CDKAL1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 22988-1-AP

  Immunofluorescent analysis of HepG2 cells using 22988-1-AP (CDKAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HepG2-Zellen, HT-1080.Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

22988-1-AP bindet in WB, IHC, IF, ELISA CDKAL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDKAL1 fusion protein Ag19060
• Vollständiger Name: CDK5 regulatory subunit associated protein 1-like 1
• Berechnetes Molekulargewicht: 579 aa, 65 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC121020
• Gene symbol: CDKAL1
• Gene ID (NCBI): 54901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell cycle progression is controlled, in part, by a family of cyclin dependent kinases (Cdks) that work to phosphorylate key substrates involved in each phase of cell cycle progression. Cdks are the catalytic subunits of serine/threonine protein kinases, a large family of proteins that act as regulators of the eukaryotic cell cycle. CDKAL1 (Cdk5 regulatory subunit associated protein 1-like 1) is a 579 amino acid single-pass membrane protein that contains one TRAM domain and is similar to Cdk5 regulatory subunit associated proteins (CDK5RAPs). Expressed in pancreas, brain and skeletal muscle, CDKAL1 uses iron as a cofactor and is involved in glucose-stimulated INS secretion. Defects in the gene encoding CDKAL1 impair INS secretion and are associated with the development of type 2 diabetes. Multiple isoforms of CDKAL1 exist due to alternative splicing events. This antibody recognizes the 65kd isoform of CDKAL1.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex; Authors - Rongsheng Huang