CDK2 Polyklonaler Antikörper

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• KD/KO Validated

CDK2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 22060-1-AP

Synonyme

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)

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Galerie der Validierungsdaten

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  WB analysis using 22060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22060-1-AP

  WB result of CDK2 antibody (22060-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and CDK2 knockout HeLa cells.

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  WB analysis using 22060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 22060-1-AP

  IP Result of anti-CDK2 (IP:22060-1-AP, 4ug; Detection:22060-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human cervical cancer using 22060-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 22060-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (22060-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 22060-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HepG2 using 22060-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

22060-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CDK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK2 fusion protein Ag17133
• Vollständiger Name: cyclin-dependent kinase 2
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003065
• Gene symbol: CDK2
• Gene ID (NCBI): 1017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919). It has 2 isoforms produced by alternative splicing with the molecular weight of 34 kDa and 30 kDa.

Publikationen

Species	Application	Title
human	IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani
human,mouse	WB,IF	Biomark Res; Long noncoding RNA HOXC-AS3 interacts with CDK2 to promote proliferation in hepatocellular carcinoma; Authors - Chen Su
human	IF	Eur J Pharmacol; Hypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.; Authors - Jinhang Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,