CDCA5 Monoklonaler Antikörper

CDCA5 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2G1D2

Kat-Nr. : 67418-1-Ig

Synonyme

CDCA5, p35, SORORIN

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Galerie der Validierungsdaten

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  WB analysis using 67418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67418-1-Ig (CDCA5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67418-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (67418-1-Ig, Clone: 2G1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

67418-1-Ig bindet in WB, IHC, IF, ELISA CDCA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDCA5 fusion protein Ag28958
• Vollständiger Name: cell division cycle associated 5
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27~35 kDa
• GenBank-Zugangsnummer: BC011000
• Gene symbol: CDCA5
• Gene ID (NCBI): 113130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).

Publikationen

Species	Application	Title
human	WB	Front Oncol; Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.; Authors - Yingji Chen
human,mouse	WB,IHC,IF	J Transl Med; TPI1 activates the PI3K/AKT/mTOR signaling pathway to induce breast cancer progression by stabilizing CDCA5.; Authors - Xiaoying Jin
human	IHC,WB	Int J Mol Sci; Identification of Candidate Genes in Breast Cancer Induced by Estrogen Plus Progestogens Using Bioinformatic Analysis; Authors - Yu Deng