CDCA2 Polyklonaler Antikörper

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• KD/KO Validated

CDCA2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17701-1-AP

Synonyme

CDCA2, Repo Man

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 17701-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17701-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17701-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17701-1-AP

  IP Result of anti-CDCA2 (IP:17701-1-AP, 4ug; Detection:17701-1-AP 1:800) with Jurkat cells lysate 2800ug.

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  IF Staining of HepG2 using 17701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17701-1-AP (CDCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

17701-1-AP bindet in WB, IP, IHC, IF, ELISA CDCA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA2 fusion protein Ag11967
• Vollständiger Name: cell division cycle associated 2
• Berechnetes Molekulargewicht: 863 aa, 95 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC036214
• Gene symbol: CDCA2
• Gene ID (NCBI): 157313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner. We got 125 kDa in western blotting maybe due to its phosphorylation.

Publikationen

Species	Application	Title
human	WB	Oncogene; CDCA2 protects against oxidative stress by promoting BRCA1-NRF2 signaling in hepatocellular carcinoma.; Authors - Shouping Wang ; KD Validated
human	WB	Oncogene; MCM8 is regulated by EGFR signaling and promotes the growth of glioma stem cells through its interaction with DNA-replication-initiating factors.; Authors - Xiaoliang Wang
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	WB,IF,IP	J Cell Sci; SUMOylation of RepoMan during late telophase regulates dephosphorylation of lamin A.; Authors - Takanobu Moriuchi ; KD Validated
human	WB,IHC	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen