CDC25C Monoklonaler Antikörper

CDC25C Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2B6B12

Rezensionen (1)

Kat-Nr. : 66912-1-Ig

Synonyme

CDC25, CDC25C, M phase inducer phosphatase 3

---

Galerie der Validierungsdaten

• 

  WB analysis using 66912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 66912-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66912-1-Ig bindet in WB, FC, ELISA CDC25C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDC25C fusion protein Ag10240
• Vollständiger Name: cell division cycle 25 homolog C (S. pombe)
• Berechnetes Molekulargewicht: 473 aa, 53 kDa
• Beobachtetes Molekulargewicht: 46-53 kDa
• GenBank-Zugangsnummer: BC019089
• Gene symbol: CDC25C
• Gene ID (NCBI): 995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Publikationen

Species	Application	Title
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
mouse	WB	Oncol Lett; Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis.; Authors - Jun Ma
human	WB	Food Chem Toxicol; DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells; Authors - Silu Hou
mouse	WB	Toxicology; ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice; Authors - Ming Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Angélique (Verified Customer) (11-25-2021)

on 20ug total proteins

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: murine hepatocytes

CDC25C Antibody Western Blot validation (1/2000 dilution) in murine hepatocytes (Cat no:66912-1-Ig)