CD90 Monoklonaler Antikörper

CD90 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

26

CloneNo.

2D7D11

Kat-Nr. : 66766-1-Ig

Synonyme

CD90, CDw90, FLJ33325, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1

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Galerie der Validierungsdaten

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  WB analysis using 66766-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 66766-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66766-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 66766-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66766-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66766-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 66766-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig colon using 66766-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66766-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66766-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Hausschwein-Hirngewebe, Hausschwein-Kolongewebe, Hausschwein-Milzgewebe, humanes Nierengewebe, Kaninchenhirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Gliomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 12 publications below
• FC: See 4 publications below

Produktinformation

66766-1-Ig bindet in WB, IHC, IF, FC, ELISA CD90 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD90 fusion protein Ag25603
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC065559
• Gene symbol: THY1
• Gene ID (NCBI): 7070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Publikationen

Species	Application	Title
human	WB	J Mol Cell Biol; Cancer-associated adipocytes-derived G-CSF promotes breast cancer malignancy via Stat3 signaling.; Authors - Li Liu
human	IHC	Int J Mol Sci; Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma.; Authors - Nobuhiko Asakura
human	FC	Int J Biol Sci; Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop.; Authors - Chong Zhou
mouse	FC	Cell Commun Signal; SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression.; Authors - Wei Sun
human	IF,WB	Cell Signal; miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.; Authors - Dong-Min Shi
mouse		Front Cardiovasc Med; Epicardium-Derived Tbx18+ CDCs Transplantation Improve Heart Function in Infarcted Mice.; Authors - Zhenglong Guo