CD90.2 Monoklonaler Antikörper

CD90.2 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus und mehr (1)

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

30-H12

Kat-Nr. : 65088-1-Ig

Synonyme

CD90, CD90.2, Thy 1, Thy 1.2, Thy1

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse thymocytes using 65088-1-Ig

  1X10^6 BALB/c mouse thymocytes were surface stained with 1 ug Anti-Mouse CD90.2 (65088-1-Ig, Clone: 30-H12) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug Rat IgG2b Isotype Control and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Thymozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65088-1-Ig bindet in FC CD90.2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: thymus cell antigen 1, theta
• GenBank-Zugangsnummer: BC054436
• Gene symbol: Thy1
• Gene ID (NCBI): 21838
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

Publikationen

Species	Application	Title
rat	FC	Regen Ther; METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3; Authors - Bin Hu