CD8a Monoklonaler Antikörper

CD8a Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

5H10-1

Kat-Nr. : 65205-1-Ig

Synonyme

BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie der Validierungsdaten

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  FC experiment of C57BL/c mouse splenocytes using 65205-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3ε and 0.25 ug Anti-Mouse CD8a (65205-1-Ig, Clone:5H10-1) and FITC anti-rat IgG2b Antibody at dilution 1:100, or rat IgG2b Isotype Control and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65205-1-Ig bindet in FC CD8a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
• Vollständiger Name: CD8 antigen, alpha chain
• GenBank-Zugangsnummer: BC030679
• Gene symbol: Cd8a
• Gene ID (NCBI): 12525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).