CD8a Polyklonaler Antikörper

CD8a Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 29896-1-AP

Synonyme

CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie der Validierungsdaten

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  WB analysis using 29896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29896-1-AP (CD8a antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse thymus using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: Maus-Thymusgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 5 publications below

Produktinformation

29896-1-AP bindet in WB, IHC, ELISA CD8a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD8a fusion protein Ag31942
• Vollständiger Name: CD8 antigen, alpha chain
• Berechnetes Molekulargewicht: 27KD
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: NM_001081110
• Gene symbol: Cd8a
• Gene ID (NCBI): 12525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients; Authors - Hualin Chen
mouse	IHC	Molecules; Immune Enhancement of Clam Peptides on Immunosuppressed Mice Induced by Hydrocortisone; Authors - Meibin Lv
mouse	IHC	J Exp Clin Cancer Res; Regnase-1 downregulation promotes pancreatic cancer through myeloid-derived suppressor cell-mediated evasion of anticancer immunity; Authors - Junya Okabe
mouse	IHC	Bioorg Chem; Discovery of selective and potent USP22 inhibitors via structure-based virtual screening and bioassays exerting anti-tumor activity; Authors - Yue Zhang
mouse	IHC	Biomed Pharmacother; FGF19/FGFR4 signaling contributes to hepatocellular carcinoma survival and immune escape by regulating IGF2BP1-mediated expression of PD-L1; Authors - Chaoqin Guo