CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

24F

Kat-Nr. : CL594-65224

Synonyme

B7 2, Cd86, CD86 molecule

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Galerie der Validierungsdaten

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  FC experiment of rat splenocytes using CL594-65224

  1X10^6 unstimulated or LPS-stimulated (5 ug/mL, 3 days) rat splenocytes were surface co-stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) and 0.5 ug CoraLite®594 Anti-Rat CD86 (CL594-65224, Clone: 24F). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS-stimulated rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65224 bindet in FC CD86 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_020081
• Gene symbol: Cd86
• Gene ID (NCBI): 56822
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.