CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

APC Fluorescent Dye

Publikationen

4

CloneNo.

GL1

Kat-Nr. : APC-65068

Synonyme

B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using APC-65068

  1X10^6 untreated (dashed lines) or LPS-treated (red) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of mouse splenocytes using APC-65068

  1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 3 publications below

Produktinformation

APC-65068 bindet in IF, FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPS-aktivierte CBA/Ca-Mausmilz-B-Zellen
• Vollständiger Name: CD86 antigen
• GenBank-Zugangsnummer: BC013807
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publikationen

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	FC	Biomed Pharmacother; Resveratrol alleviated neuroinflammation induced by pseudorabies virus infection through regulating microglial M1/M2 polarization; Authors - Xiangxiu Chen
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu
mouse	FC	Stem Cells Dev; Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice; Authors - Hongbing Lin