CD82 Monoklonaler Antikörper

CD82 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1E7A4

Rezensionen (1)

Kat-Nr. : 66803-1-Ig

Synonyme

4F9, C33, C33 antigen, CD82, CD82 antigen, CD82 molecule, GR15, IA4, Inducible membrane protein R2, KAI1, Metastasis suppressor Kangai 1, R2, SAR2, ST6, Tetraspanin 27, Tspan 27, TSPAN27

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Galerie der Validierungsdaten

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  WB analysis using 66803-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66803-1-Ig (CD82 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66803-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66803-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66803-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66803-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66803-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66803-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66803-1-Ig bindet in WB, IHC, IF, ELISA CD82 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD82 fusion protein Ag17513
• Vollständiger Name: CD82 molecule
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000726
• Gene symbol: CD82
• Gene ID (NCBI): 3732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Publikationen

Species	Application	Title
human	IHC	J Proteomics; Proteomic profiling of plasma exosomes reveals CD82 involvement in the development of esophageal squamous cell carcinoma.; Authors - Lifei Yang
human	WB	FEBS Open Bio; RAB31 in glioma-derived endothelial cells promotes glioma cell invasion via extracellular vesicle-mediated enrichment of MYO1C; Authors - Jinghao Suo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

CD82 Antibody Western Blot validation (1:1000 dilution) in ARPE-19 (Cat no:66803-1-Ig)