CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

3H5

Kat-Nr. : 65217-1-Ig

Synonyme

B7 1, Cd80, Cd80 molecule

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Galerie der Validierungsdaten

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  FC experiment of wistar rat splenocytes using 65217-1-Ig

  1X10^6 unstimulated (dashed) or LPS-stimulated Wistar rat splenocytes were surface stained with 1 ug Anti-Rat CD80 (65217-1-Ig, Clone:3H5) and FITC anti-Mouse IgG1 Antibody at dilution 1:100 (red), or 1 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS-stimulated Wistar rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65217-1-Ig bindet in FC CD80 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Vollständiger Name: Cd80 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: XM_017597885
• Gene symbol: Cd80
• Gene ID (NCBI): 25408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)