CD79B Polyklonaler Antikörper

CD79B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21063-1-AP

Synonyme

B29, CD79B, Ig beta, IGB

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Galerie der Validierungsdaten

• 

  WB analysis of Raji using 21063-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21063-1-AP (CD79B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Maus-Kolongewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

21063-1-AP bindet in WB, IHC, IF, ELISA CD79B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD79B fusion protein Ag15086
• Vollständiger Name: CD79b molecule, immunoglobulin-associated beta
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC032651
• Gene symbol: CD79B
• Gene ID (NCBI): 974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Front Immunol; CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.; Authors - Zhenghao Wu
mouse	IF	Cell Discov; Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure; Authors - Jie Yin