CD73 Rekombinanter Antikörper

CD73 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

AD2

Kat-Nr. : 65500-1-RR

Synonyme

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) (left) or 0.4 ug Anti-Human CD73 Mouse Monoclonal Antibody (Clone:AD2) (center and right). Each unconjugated primary antibody was labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) or FlexAble CL750 Antibody Labeling Kit for Mouse IgG1 (KFA024) prior to staining. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65500-1-RR bindet in FC CD73 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Pre-B leukemia cell line
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: NT5E
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).