CD71 Monoklonaler Antikörper

CD71 Monoklonal Antikörper für IHC, WB

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC

Konjugation

Unkonjugiert

CloneNo.

H68.4

Kat-Nr. : 65236-1-Ig

Synonyme

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Galerie der Validierungsdaten

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  WB analysis using 65236-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 65236-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 65236-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 65236-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, K-562-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65236-1-Ig bindet in WB, IHC CD71 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: TFRC
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.