CD69 Monoklonaler Antikörper

CD69 Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

H1.2F3

Kat-Nr. : CL555-65105

Synonyme

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 unstimulated or anti-CD3/CD28-stimulated mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) or 0.5 ug Isotype Control. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28-stimulated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-65105 bindet in FC CD69 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus dendritische epidermale T-Zelllinie Y245
• Vollständiger Name: CD69 antigen
• GenBank-Zugangsnummer: BC106997
• Gene symbol: Cd69
• Gene ID (NCBI): 12515
• Konjugation: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).