CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human und mehr (1)

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

Y1/82A

Kat-Nr. : 65187-1-Ig

Synonyme

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Galerie der Validierungsdaten

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  IF Staining of THP-1 using 65187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using 65187-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: THP-1-Zellen
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

65187-1-Ig bindet in IF, FC CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysosomaler Inhalt von Lungenmakrophagen
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publikationen

Species	Application	Title
mouse	IF	Int Immunopharmacol; Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway; Authors - Xingchen Xiang
mouse	IF	Phytomedicine; Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice; Authors - Gui-Lin Jin
mouse	FC	Int Immunopharmacol; Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis; Authors - Qiuyuan Shao
human	IF	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu
mouse	IF	Exp Neurol; Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model; Authors - Juan Chen