CD62L (L-Selectin) Monoklonaler Antikörper

CD62L (L-Selectin) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

MEL-14

Kat-Nr. : 65123-1-Ig

Synonyme

CD62L (L-Selectin), Sell

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using 65123-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Anti-Mouse CD62L (L-Selectin) (65123-1-Ig, Clone: MEL-14) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65123-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65123-1-Ig bindet in FC CD62L (L-Selectin) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/eb-Maus-B-Lymphom 38C-13
• Vollständiger Name: selectin, lymphocyte
• GenBank-Zugangsnummer: BC052681
• Gene symbol: Sell
• Gene ID (NCBI): 20343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Publikationen

Species	Application	Title
mouse	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang