CD62L Monoklonaler Antikörper
CD62L Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
DREG56
Kat-Nr. : CL488-65167
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | humane PBMCs |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-65167 bindet in FC CD62L und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG1, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | PMA-aktivierte humane Leukozyten aus peripherem Blut |
Vollständiger Name | selectin L |
Berechnetes Molekulargewicht | 42 kDa |
GenBank-Zugangsnummer | BC020758 |
Gene symbol | SELL |
Gene ID (NCBI) | 6402 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Mit Protein-A-Affinitätschromatographie gereinigt |
Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
Protokolle
Produktspezifische Protokolle | |
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FC protocol for CL Plus 488 CD62L antibody CL488-65167 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |