CD61 Monoklonaler Antikörper

CD61 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

VIPL2

Kat-Nr. : APC-65185

Synonyme

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood platelets using APC-65185

  1X10^6 human peripheral blood platelets were surface stained with 10.00 ul/test APC Anti-Human CD61 (APC-65185, Clone:VIPL2) (red) or isotype control antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood platelets

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65185 bindet in FC CD61 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• GenBank-Zugangsnummer: BC127666
• Gene symbol: ITGB3
• Gene ID (NCBI): 3690
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).