CD59 Monoklonaler Antikörper

CD59 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E3A1

Kat-Nr. : 68222-1-Ig

Synonyme

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

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Galerie der Validierungsdaten

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  WB analysis using 68222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 68222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 68222-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human placenta using 68222-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of BxPC-3 using 68222-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 68222-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD59 (68222-1-Ig, Clone:1E3A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolostrum, A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Plazenta-Gewebe, BxPC-3-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68222-1-Ig bindet in WB, IHC, IF, FC, ELISA CD59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD59 fusion protein Ag32834
• Vollständiger Name: CD59 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC001506
• Gene symbol: CD59
• Gene ID (NCBI): 966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.