CD54 Monoklonaler Antikörper

CD54 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

YN1/1.7.4

Kat-Nr. : CL488-65066

Synonyme

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL488-65066

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Mouse CD54 (CL488-65066, Clone: YN1/1.7.4) (red) or isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65066 bindet in FC CD54 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-NS-1-Zellen
• Vollständiger Name: intercellular adhesion molecule 1
• GenBank-Zugangsnummer: BC008626
• Gene symbol: Icam1
• Gene ID (NCBI): 15894
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).