CD49b Monoklonaler Antikörper

CD49b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgM, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

1

CloneNo.

DX5

Kat-Nr. : APC-65065

Synonyme

CD49B, DX5, integrin alpha 2, Itga2

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC Anti-Mouse CD49b (APC-65065, Clone: DX5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC-Rat IgM isotype control antibody. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

APC-65065 bindet in FC CD49b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgM, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aus C57BL/6-Mäusen isolierte NK-Zellen
• Vollständiger Name: integrin alpha 2
• GenBank-Zugangsnummer: BC065139
• Gene symbol: Itga2
• Gene ID (NCBI): 16398
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und einem Stabilisator.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.

Publikationen

Species	Application	Title
	FC	Liver Int; Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury; Authors - Yuan Li